Western blot analysis of various lysates, using PARVA Rabbit pAb (CAB24474) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The PARVA Polyclonal Antibody (CAB24474) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human Parva and is validated for use in various applications, including Western blotting. By binding to the Parva protein, researchers can accurately detect and analyze its expression in different cell types, making it an essential tool for studies in cell biology and cancer research.Parva, also known as alpha-actinin-1, plays a crucial role in maintaining cell structure and facilitating cell movement. Its involvement in cell adhesion and cytoskeletal organization makes it a target of interest for research on processes such as cell migration and invasion.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PARVA Polyclonal Antibody
SKU:
CAB24474
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PARVA, CH-ILKBP, MXRA2, alpha-parvin
Positive Sample:
MCF7, HT-29
Calculated MW:
20kDa/42kDa
Observed MW:
45-50kDa
This gene encodes a member of the parvin family of actin-binding proteins. Parvins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. The encoded protein is part of the integrin-linked kinase signaling complex and plays a role in cell adhesion, motility and survival.
Purification Method
Affinity purification
Gene ID
55742
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using PARVA Rabbit pAb (CAB24474) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
