Anti-PAX3 Antibody (CAB13930)

Antibody Name:	Anti-PAX3 Antibody
Antibody SKU:	CAB13930
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human PAX3 (NP_039230.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human PAX3 (NP_039230.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTTL AGAV PRMM RPGP GQNY PRSG FPLE VSTP LGQG RVNQ LGGV FING RPLP NHIR HKIV EMAH HGIR PCVI SRQL RVSH GCVS KILC RYQE TGSI RPGA IGGS KPKQ VTTP DVEK KIEE YKRE NPGM FSWE IRDK LLKD AVCD RNTV PSVS SISR ILRS KFGK GEEE EADL ERKE AEES EKKA KHSI DGIL SERG KALV SGVS SH
Gene ID:	5077
Uniprot:	P23760
Cellular Location:	Nucleus
Calculated MW:	22kDa/24kDa/44kDa/45kDa/52kDa/53kDa/55kDa
Observed MW:	65kDa

Synonyms:

PAX3, CDHS, HUP2, WS1, WS3

Background:

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.

UniProt Protein Function:	PAX3: Probable transcription factor associated with development of alveolar rhabdomyosarcoma. Can bind to DNA as a heterodimer with PAX7. Interacts with DAXX. Belongs to the paired homeobox family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; Oncoprotein; DNA-binding Chromosomal Location of Human Ortholog: 2q35 Cellular Component: nucleoplasm Molecular Function:protein binding; sequence-specific DNA binding Biological Process: apoptosis; organ morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; sensory perception of sound; transcription from RNA polymerase II promoter Disease: Craniofacial-deafness-hand Syndrome; Rhabdomyosarcoma 2; Waardenburg Syndrome, Type 1; Waardenburg Syndrome, Type 3
NCBI Summary:	This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008]
UniProt Code:	P23760
NCBI GenInfo Identifier:	1172022
NCBI Gene ID:	5077
NCBI Accession:	P23760.2
UniProt Secondary Accession:	P23760,Q16448, Q494Z3, Q494Z4, Q53T90, Q6GSJ9, Q86UQ2 Q86UQ3, G5E9C1,
UniProt Related Accession:	P23760
Molecular Weight:	55,975 Da
NCBI Full Name:	Paired box protein Pax-3
NCBI Synonym Full Names:	paired box 3
NCBI Official Symbol:	PAX3
NCBI Official Synonym Symbols:	WS1; WS3; CDHS; HUP2
NCBI Protein Information:	paired box protein Pax-3
UniProt Protein Name:	Paired box protein Pax-3
UniProt Synonym Protein Names:	HuP2
Protein Family:	Paired box protein
UniProt Gene Name:	PAX3
UniProt Entry Name:	PAX3_HUMAN

	Western blot analysis of extracts of 293T cells, using PAX3 antibody (CAB13930) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human lung cancer using PAX3 antibody (CAB13930) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using PAX3 antibody (CAB13930) at dilution of 1:100 (40x lens).

Antibodies

Anti-PAX3 Antibody (CAB1675)

PAX3 Antibody (PACO11081)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)