The Paxillin Monoclonal Antibody (CAB19100) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards paxillin in human samples, making it an ideal choice for Western blotting experiments. By binding to paxillin, this antibody enables accurate detection and analysis of paxillin levels in various cell types.Paxillin is a key player in cell adhesion and migration processes, making it an important target for studies in cancer metastasis, developmental biology, and tissue remodeling.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Paxillin Monoclonal Antibody
SKU:
CAB19100
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0368
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445).
Purification Method
Affinity purification
Gene ID
5829
RRID
AB_2862593
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Paxillin Rabbit mAb (CAB19100) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from HeLa cells, using Paxillin Rabbit mAb (CAB19100) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
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