Anti-PCNA Antibody (CAB9909)

Antibody Name:	Anti-PCNA Monoclonal Antibody
Antibody SKU:	CAB9909
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Mouse
Immunogen:	Recombinant protein of human PCNA

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, A-431, 293T, NIH/3T3, PC-12, Mouse spleen, Rat testis

Immunogen:	Recombinant protein of human PCNA
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	5111
Uniprot:	P12004
Cellular Location:	Nucleus
Calculated MW:	28kDa
Observed MW:	28kDa

Synonyms:

PCNA, ATLD2

Background:

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

UniProt Protein Function:	PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.
UniProt Protein Details:	Protein type:DNA replication; Cell cycle regulation Chromosomal Location of Human Ortholog: 20pter-p12 Cellular Component: nucleoplasm; centrosome; PCNA complex; nuclear replication fork; cytoplasm; DNA replication factor C complex; nucleus Molecular Function:identical protein binding; protein binding; DNA polymerase processivity factor activity; MutLalpha complex binding; purine-specific mismatch base pair DNA N-glycosylase activity; receptor tyrosine kinase binding; dinucleotide insertion or deletion binding Biological Process: mismatch repair; heart development; positive regulation of deoxyribonuclease activity; DNA strand elongation during DNA replication; response to lipid; DNA repair; leading strand elongation; G1/S-specific transcription in mitotic cell cycle; telomere maintenance via semi-conservative replication; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; regulation of DNA replication; G1/S transition of mitotic cell cycle Disease: Ataxia-telangiectasia-like Disorder 2
NCBI Summary:	The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
UniProt Code:	P12004
NCBI GenInfo Identifier:	129694
NCBI Gene ID:	5111
NCBI Accession:	P12004.1
UniProt Secondary Accession:	P12004,B2R897, D3DW02,
UniProt Related Accession:	P12004
Molecular Weight:	261
NCBI Full Name:	Proliferating cell nuclear antigen
NCBI Synonym Full Names:	proliferating cell nuclear antigen
NCBI Official Symbol:	PCNA
NCBI Official Synonym Symbols:	ATLD2
NCBI Protein Information:	proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
UniProt Protein Name:	Proliferating cell nuclear antigen
UniProt Synonym Protein Names:	Cyclin
Protein Family:	PCNA-associated factor
UniProt Gene Name:	PCNA
UniProt Entry Name:	PCNA_HUMAN

	Western blot analysis of extracts of various cell lines, using PCNA antibody (CAB9909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human colon using PCNA Mouse mAb (CAB9909) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-PCNA Antibody (CAB0264)	Human PCNA / Proliferating Cell Nuclear ELISA Kit
Anti-PCNA Antibody (CAB13336)	Human PCNA (Proliferating Cell Nuclear Antigen) CLIA Kit (HUES01246)
Anti-PCNA Antibody (CAB12427)	Human Proliferating cell nuclear antigen (PCNA) ELISA Kit
PCNA Antibody (PACO06738)
PCNA Antibody (PACO11108)

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)