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PCS (Pentachlorophenol Sodium) ELISA Kit (FSES0065)

SKU:
FSES0065
Product Type:
ELISA Kit
Applications:
ELISA
Research Area:
Food Science
€599
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Description

Technical Manual

PCS (Pentachlorophenol Sodium) ELISA Kit

Kit Name: PCS (Pentachlorophenol Sodium) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Pentachlorophenol Sodium (PCS) in samples, such as tissues, feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, PCS in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-PCS antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of PCS. The concentration of PCS in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.2 ppb (ng/mL)
Reaction Mode: 25°C,30 min~15 min
Detection Limit: Tissue,Feed ---1 ppb.
Cross Reactivity: Pentachlorophenol Sodium---100%; DDT, Hexachlorocyclohexane, Dicofol --- <0.1%
Sample Recovery Rate: 90% ± 30%.
Kits Components:
Item Specifications
ELISA Microtiter Plate 96 wells
Standard Liquid 1.5 mL each (0 ppb, 0.2 ppb, 0.6 ppb, 1.8 ppb, 5.4 ppb, 16.2 ppb)
11×Concentrated HRP ConjugateSolution 1 mL
HRP Conjugate Dilution 7 mL
Substrate Reagent A 7 mL
Substrate Reagent B 7 mL
Stop Solution 7 mL
20×Concentrated Wash Buffer 25 mL
4×Concentrated Sample Solution 50 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate Reader, Homogenizer, Oscillators, Nitrogen Evaporators, Water bath, Centrifuge, Balance (sensibility 0.01g).
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: Ethyl Acetate, N-hexane.
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.