Anti-PDCD6 Antibody (CAB6685)

Antibody Name:	Anti-PDCD6 Antibody
Antibody SKU:	CAB6685
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human PDCD6 (NP_037364.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, HeLa, SW620, HepG2, Mouse liver, Mouse kidney, Mouse lung, Mouse ovary, Rat ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human PDCD6 (NP_037364.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAY SYRP GPGA GPGP AAGA ALPD QSFL WNVF QRVD KDRS GVIS DTEL QQAL SNGT WTPF NPVT VRSI ISMF DREN KAGV NFSE FTGV WKYI TDWQ NVFR TYDR DNSG MIDK NELK QALS GFGY RLSD QFHD ILIR KFDR QGRG QIAF DDFI QGCI VLQR LTDI FRRY DTDQ DGWI QVSY EQYL SMVF SIV
Gene ID:	10016
Uniprot:	O75340
Cellular Location:	Endoplasmic reticulum membrane, Endosome, Nucleus, Nucleus membrane, Peripheral membrane protein
Calculated MW:	7kDa/21kDa
Observed MW:	16kDa

Synonyms:

PDCD6, ALG-2, ALG2, PEF1B

Background:

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5.

UniProt Protein Function:	PDCD6: Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)- regulated signals along the death pathway. Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 5p15.33 Cellular Component: endoplasmic reticulum membrane; nuclear membrane; endoplasmic reticulum; cytoplasm; cytoplasmic vesicle; nucleus; endosome Molecular Function:protein dimerization activity; protein binding; protein homodimerization activity; protein anchor; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; calcium-dependent protein binding; molecular adaptor activity Biological Process: caspase activation; intracellular protein transport; negative regulation of TOR signaling pathway; positive regulation of angiogenesis; negative regulation of protein kinase B signaling cascade; negative regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of caspase activity; positive regulation of endothelial cell proliferation; angiogenesis; proteolysis; response to calcium ion
NCBI Summary:	This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5. [provided by RefSeq, May 2012]
UniProt Code:	O75340
NCBI GenInfo Identifier:	12230420
NCBI Gene ID:	10016
NCBI Accession:	O75340.1
UniProt Secondary Accession:	O75340,Q2YDC2, Q5TZS0, B2RD16, E7ESR3,
UniProt Related Accession:	O75340
Molecular Weight:	191
NCBI Full Name:	Programmed cell death protein 6
NCBI Synonym Full Names:	programmed cell death 6
NCBI Official Symbol:	PDCD6
NCBI Official Synonym Symbols:	ALG2; ALG-2; PEF1B
NCBI Protein Information:	programmed cell death protein 6; apoptosis-linked gene 2 protein; probable calcium-binding protein ALG-2
UniProt Protein Name:	Programmed cell death protein 6
UniProt Synonym Protein Names:	Apoptosis-linked gene 2 protein; Probable calcium-binding protein ALG-2
Protein Family:	Programmed cell death protein
UniProt Gene Name:	PDCD6
UniProt Entry Name:	PDCD6_HUMAN

	Western blot analysis of extracts of various cell lines, using PDCD6 antibody (CAB6685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using PDCD6 antibody (CAB6685) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of HeLa cells using PDCD6 antibody (CAB6685). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug PDCD6 antibody (CAB6685). Western blot was performed from the immunoprecipitate using PDCD6 antibody (CAB6685) at a dilition of 1:1000.

Antibodies

Anti-PDCD6 Antibody (CAB19924)[KO Validated]

PDCD6 Antibody (PACO11129)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)