Anti-PDE6D Antibody (CAB14762)

Antibody Name:	Anti-PDE6D Antibody
Antibody SKU:	CAB14762
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PDE6D (NP_002592.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, OVCAR3, Mouse brain, Mouse kidney, Mouse lung, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PDE6D (NP_002592.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSAK DERA REIL RGFK LNWM NLRD AETG KILW QGTE DLSV PGVE HEAR VPKK ILKC KAVS RELN FSST EQME KFRL EQKV YFKG QCLE EWFF EFGF VIPN STNT WQSL IEAA PESQ MMPA SVLT GNVI IETK FFDD DLLV STSR VRLF YV
Gene ID:	5147
Uniprot:	O43924
Cellular Location:	Cytoplasm, Cytoplasmic vesicle membrane, Peripheral membrane protein, cilium basal body, cytoskeleton, cytosol
Calculated MW:	17kDa
Observed MW:	14-15kDa

Synonyms:

PDE6D, JBTS22, PDED

Background:

This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants.

UniProt Protein Function:	PDE6D: Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. Belongs to the PDE6D/unc-119 family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 2q35-q36 Cellular Component: cytoplasmic vesicle; cytosol Molecular Function:GTPase inhibitor activity; protein binding; Rab GTPase binding Disease: Joubert Syndrome 22
NCBI Summary:	This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]
UniProt Code:	O43924
NCBI GenInfo Identifier:	5921809
NCBI Gene ID:	5147
NCBI Accession:	O43924.1
UniProt Secondary Accession:	O43924,O43250,
UniProt Related Accession:	O43924
Molecular Weight:	17,420 Da
NCBI Full Name:	Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta
NCBI Synonym Full Names:	phosphodiesterase 6D
NCBI Official Symbol:	PDE6D
NCBI Official Synonym Symbols:	PDED; JBTS22
NCBI Protein Information:	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta
UniProt Protein Name:	Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta
UniProt Synonym Protein Names:	Protein p17
Protein Family:	Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase
UniProt Gene Name:	PDE6D
UniProt Entry Name:	PDE6D_HUMAN

	Western blot analysis of extracts of various cell lines, using PDE6D antibody (CAB14762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of L929 cells using PDE6D Polyclonal Antibody (CAB14762) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using PDE6D Polyclonal Antibody (CAB14762) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)