The CD31/PECAM1 Antibody (CAB2104) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications. By binding to the PECAM1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in vascular biology and immunology.PECAM1 plays a crucial role in various physiological processes, including leukocyte trafficking, vascular remodeling, and angiogenesis. Its involvement in cellular interactions and signal transduction pathways makes it a key target for research on cardiovascular diseases, inflammation, and cancer.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD31/PECAM1 Antibody
SKU:
CAB2104
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Jurkat, Mouse lung, Rat spleen, Rat lung, Rat thymus, Mouse spleen
Cellular Localization:
Cell Junction, Cell Junction, Cell Membrane, Lipid-Anchor, Single-Pass Type I Membrane Protein.
Calculated MW:
83kDa
Observed MW:
130kDa/110-135kDa
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Purification Method
Affinity purification
Gene ID
5175
RRID
AB_2764123
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using CD31/PECAM1 Rabbit pAb (CAB2104) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using CD31/PECAM1 Rabbit pAb (CAB2104) at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of paraffin-embedded human placenta using CD31/PECAM1 Rabbit pAb (CAB2104) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
