Anti-PEG10 Antibody (CAB2787)

Antibody Name:	Anti-PEG10 Antibody
Antibody SKU:	CAB2787
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human PEG10 (NP_001035242.1).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, SW620, HepG2, U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human PEG10 (NP_001035242.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTER RRDE LSEE INNL REKV MKQS EENN NLQS QVQK LTEE NTTL REQV EPTP EDED DDIE LRGA AAAA APPP PIEE ECPE DLPE KFDG NPDM LAPF MAQC QIFM EKST RDFS VDRV RVCF VTSM MTGR AARW ASAK LERS HYLM HNYP AFMM EMKH VFED PQRR EVAK RKIR RLRQ GMGS VIDY SNAF QMIA QDLD WNEP ALID QYHE GLSD HIQE ELSH LEVA KSLS ALIG QCIH IERR LARA AAAR KPRS PPRA LVLP HIAS HHQV DPTE PVGG ARMR LTQE EKER RRKL NLCL YCGT GGHY ADNC PAKA SKSS PAGN SPAP L
Gene ID:	23089
Uniprot:	Q86TG7
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	36kDa/80kDa
Observed MW:	55kDa

Synonyms:

PEG10, EDR, HB-1, MEF3L, Mar2, Mart2, RGAG3, RTL2, SIRH1

Background:

This is a paternally expressed imprinted gene that is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1; and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by -1 translational frameshifting (-1 FS). While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for -1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation and apoptosis. Overexpression of this gene has been associated with several malignancies, such as hepatocellular carcinoma and B-cell lymphocytic leukemia. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. Additional isoforms resulting from alternatively spliced transcript variants, and use of upstream non-AUG (CUG) start codon have been reported for this gene.

UniProt Protein Function:	PEG10: Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation. May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter. Up-regulated by MYC and dihydrotestosteron (DHT). Interacts with ALK1, SIAH1 and SIAH2. 2 isoforms of the human protein are produced by ribosomal frameshifting.
UniProt Protein Details:	Protein type:RNA-binding; Cell cycle regulation; Apoptosis; Cell development/differentiation Chromosomal Location of Human Ortholog: 7q21 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding; DNA binding; zinc ion binding Biological Process: apoptosis; cell differentiation; negative regulation of transforming growth factor beta receptor signaling pathway
UniProt Code:	Q86TG7
NCBI GenInfo Identifier:	172046699
NCBI Gene ID:
NCBI Accession:	Q86TG7.2
Molecular Weight:	55kDa
NCBI Full Name:	Retrotransposon-derived protein PEG10
UniProt Protein Name:	Retrotransposon-derived protein PEG10
UniProt Synonym Protein Names:	Embryonal carcinoma differentiation-regulated protein; Mammalian retrotransposon-derived protein 2; Myelin expression factor 3-like protein 1; MEF3-like protein 1; Paternally expressed gene 10 protein; Retrotransposon gag domain-containing protein 3; Retrotransposon-derived gag-like polyprotein; Ty3/Gypsy-like protein
Protein Family:	Retrotransposon-derived protein
UniProt Gene Name:	PEG10
UniProt Entry Name:	PEG10_HUMAN

	Western blot analysis of extracts of various cell lines, using PEG10 antibody (CAB2787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat testis using PEG10 Rabbit pAb (CAB2787) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using PEG10 Rabbit pAb (CAB2787) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using PEG10 Rabbit pAb (CAB2787) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using PEG10 Rabbit pAb (CAB2787) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
PEG10 Antibody (PACO11164)	Human PEG10 / EDR ELISA Kit
	Human Retrotransposon-derived protein PEG10 (PEG10) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)