Description
PER1 Rabbit Polyclonal Antibody (CAB8449)
The PER1 Rabbit Polyclonal Antibody (CAB8449) from Assay Genie is a rabbit polyclonal antibody for the detection of PER1 in human, mouse and rat samples. It is validated for Western blot (WB), immunohistochemistry on paraffin-embedded tissues (IHC-P), immunofluorescence/immunocytochemistry (IF/ICC) and ELISA, and is affinity purified. This antibody is supplied as a kit for advanced applications; the kit includes Bradford Reagent to quantify total protein concentration for accurate sample normalization (optional). Manufactured and quality-released by Assay Genie Limited.
| Product Name: | PER1 Rabbit Polyclonal Antibody |
| SKU: | CAB8449 |
| Size: | 100 µL or 20 µL |
| Category: | Polyclonal Antibody |
| Species: | Rabbit |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant protein (or fragment). | ||||||||
| Tested Applications: | WBIHC-PIF/ICC ELISA | ||||||||
| Recommended Dilution: |
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| Synonyms: | PER; hPER; RIGUI; PER1 |
| Calculated MW: | 136 kDa / 92 kDa |
| Observed MW: | 136 kDa |
| Purification Method | Affinity purification |
| Gene ID | 5187 |
| Uniprot | O15534 |
| Storage | Store at -20 °C. Shelf life: 12 months. |
| Shipping | Gel Pack |
 | Western blot analysis of lysates from Mouse brain, using PER1 Rabbit Polyclonal Antibody (CAB8449) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60 s. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PER1 Rabbit Polyclonal Antibody (CAB8449) at dilution of 1:100 (40x lens). High-pressure antigen retrieval performed with 0.01 M Citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of HeLa cells using PER1 Rabbit Polyclonal Antibody (CAB8449) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using PER1 Rabbit Polyclonal Antibody (CAB8449) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2OS cells using PER1 Rabbit Polyclonal Antibody (CAB8449) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. |
