Anti-PFKL Antibody (CAB7708)

Antibody Name:	Anti-PFKL Antibody
Antibody SKU:	CAB7708
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 700-780 of human PFKL (NP_002617.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	A-549, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 700-780 of human PFKL (NP_002617.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FANA PDSA CVIG LKKK AVAF SPVT ELKK DTDF EHRM PREQ WWLS LRLM LKML AQYR ISMA AYVS GELE HVTR RTLS MDKG F
Gene ID:	5211
Uniprot:	P17858
Cellular Location:	Cytoplasm
Calculated MW:	85kDa/90kDa
Observed MW:	85kDa

Synonyms:	PFKL, ATP-PFK, PFK-B, PFK-L
Background:	This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1, 6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	PFKL: phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Kinase, other; EC 2.7.1.11; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: 6-phosphofructokinase complex; cytosol; membrane Molecular Function:6-phosphofructokinase activity; ATP binding; identical protein binding; kinase binding; protein binding Biological Process: fructose 1,6-bisphosphate metabolic process; fructose 6-phosphate metabolic process; glycolysis; protein oligomerization; response to glucose stimulus
NCBI Summary:	This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
UniProt Code:	P17858
NCBI GenInfo Identifier:	134048493
NCBI Gene ID:	5211
NCBI Accession:	P17858.6
UniProt Secondary Accession:	P17858,Q96A64, Q96IH4, Q9BR91,
UniProt Related Accession:	P17858
Molecular Weight:	90,203 Da
NCBI Full Name:	ATP-dependent 6-phosphofructokinase, liver type
NCBI Synonym Full Names:	phosphofructokinase, liver type
NCBI Official Symbol:	PFKL
NCBI Official Synonym Symbols:	PFK-B; PFK-L; ATP-PFK
NCBI Protein Information:	ATP-dependent 6-phosphofructokinase, liver type
UniProt Protein Name:	ATP-dependent 6-phosphofructokinase, liver type
UniProt Synonym Protein Names:	6-phosphofructokinase type B; Phosphofructo-1-kinase isozyme B; PFK-B; Phosphohexokinase
Protein Family:	ATP-dependent 6-phosphofructokinase
UniProt Gene Name:	PFKL
UniProt Entry Name:	PFKAL_HUMAN

	Western blot analysis of extracts of various cell lines, using PFKL antibody (CAB7708) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of NIH/3T3 cells using PFKL antibody (CAB7708) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using PFKL antibody (CAB7708) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-PFKL Antibody (CAB15080)	Human 6-phosphofructokinase, liver type (PFKL) ELISA Kit
PFKL Antibody (PACO06743)
PFKL Antibody (PACO11185)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)