The PGAM5 Antibody (CAB16022) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables GTPase activator activity and protein serine/threonine phosphatase activity. Involved in necroptotic process. Located in mitochondrion.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PGAM5 Antibody
SKU:
CAB16022
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BXLBV68, PGAM5
Positive Sample:
HeLa, A-549, MCF7
Cellular Localization:
Mitochondrial Outer Membrane, Mitochondrion.
Calculated MW:
32kDa
Observed MW:
28kDa/30kDa
Enables GTPase activator activity and protein serine/threonine phosphatase activity. Involved in necroptotic process. Located in mitochondrion.
Purification Method
Affinity purification
Gene ID
192111
RRID
AB_2763459
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using PGAM5 Rabbit pAb (CAB16022) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using PGAM5 Rabbit pAb (CAB16022) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using PGAM5 Rabbit pAb (CAB16022) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of MCF7 cells using PGAM5 Rabbit pAb (CAB16022) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
