Western blot analysis of extracts from normal (control) and PHD2/EGLN1 knockout (KO) 293T(KO) cells, using PHD2/EGLN1 antibody (CAB21252) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The PHD2/EGLN1 Monoclonal Antibody (CAB21252) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, generated through hybridoma technology, offers high specificity and sensitivity for detecting PHD2/EGLN1 in human samples, making it ideal for use in immunohistochemistry, immunofluorescence, and other experimental techniques.PHD2/EGLN1 is a member of the prolyl hydroxylase domain protein family and is involved in the hydroxylation of hypoxia-inducible factor (HIF), a transcription factor that regulates the expression of genes involved in oxygen homeostasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PHD2/EGLN1 Monoclonal Antibody
SKU:
CAB21252
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC52542
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MAND SGGP GGPS PSER DRQY CELC GKME NLLR CSRC RSSF YCCK EHQR QDWK KHKL VCQG SEGA LGHG VGPH QHSG PAPP AAVP PPRA GARE PRKA AARR
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:20000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
Purification Method
Affinity purification
Gene ID
54583
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and PHD2/EGLN1 knockout (KO) 293T cells, using [KO Validated] PHD2/EGLN1 Rabbit mAb (CAB21252) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 8s.
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