The Phospho-Axl-Y702 Polyclonal Antibody (CABP0848) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is specifically designed to target the phosphorylated form of AXL at tyrosine 702, which plays a crucial role in AXL signaling pathways.The antibody is highly reactive with human samples and has been validated for use in Western blot applications, allowing for the detection and analysis of phospho-AXL in different cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-Axl-Y702 Polyclonal Antibody
SKU:
CABP0848
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
GDYY R
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ARK, UFO, JTK11, Tyro7, Phospho-Axl-Y702
Positive Sample:
A-549 treated with Gas6
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
98kDa
Observed MW:
138kDa
The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. The encoded protein acts as a host cell receptor for multiple viruses, including Marburg, Ebola and Lassa viruses and is a candidate receptor for the SARS-CoV2 virus.
Purification Method
Affinity purification
Gene ID
558
RRID
AB_2770931
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-549 cells, using Phospho-Axl-Y702 Rabbit pAb (CABP0848) at 1:400 dilution. A-549 cells were treated with Gas6 (400 ng/ml) at 37℃ for 5 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
