The Phospho-Tau-T212 Polyclonal Antibody (CABP0400) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbits as the host species, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By specifically targeting the phosphorylated form of MAPT at threonine 212, this antibody allows for the detection and analysis of MAPT in various cell types, making it an excellent choice for studies in neuroscience and neurodegenerative diseases.MAPT, also known as tau protein, plays a crucial role in stabilizing microtubules and facilitating intracellular transport within neurons.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-Tau-T212 Polyclonal Antibody
SKU:
CABP0400
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Purification Method
Affinity purification
Gene ID
4137
RRID
AB_2771606
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-Tau-T212 Rabbit pAb (CABP0400) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
