The Phospho-CDC37-S13 Monoclonal Antibody (CABP1141) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, meticulously developed using rabbit monoclonal technology, exhibits high specificity and sensitivity towards human samples, making it suitable for a range of applications including Western blotting.CDC37 is a molecular chaperone that plays a crucial role in the stabilization and activation of client protein kinases involved in cell growth and proliferation. Phosphorylation of CDC37 at serine 13 is known to modulate its interaction with client kinases, thereby influencing their activity and downstream signaling cascades.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-CDC37-S13 Monoclonal Antibody
SKU:
CABP1141
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1602
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
EVSD D
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P50CDC37, Phospho-CDC37-S13
Positive Sample:
293T, NIH/3T3, C6
Cellular Localization:
Cytoplasm.
Calculated MW:
44kDa
Observed MW:
50kDa
The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.
Purification Method
Affinity purification
Gene ID
11140
RRID
AB_2864008
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-CDC37-S13 Rabbit mAb (CABP1141) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from C6 cells, using Phospho-CDC37-S13 Rabbit mAb (CABP1141) at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
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