null

Phospho-C/EBPB-T235 Rabbit Polyclonal Antibody (CABP1055)

SKU:
CABP1055
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-C/EBPB-T235 Rabbit Polyclonal Antibody
SKU:CABP1055
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around T235 of human C/EBPB (NP_005185.2).
Sequence:PGTP S
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:TCF5; IL6DBP; NF-IL6; C/EBP-beta; Phospho-C/EBPB-T235
Positive Sample:NIH/3T3
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:36kDa
Observed MW:38kDa

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.

Purification Method:Affinity purification
Gene ID:1051
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of NIH/3T3, using Phospho-C/EBPB-T235 antibody (CABP1055) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.NIH/3T3 cells were treated by Serum-starvation overnight at 37℃.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

Related Products