The Phospho-CRMP2/DPYSL2-T514 Antibody (CABP0821) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for phosphorylated DPYSL2 at threonine 514 and is suitable for use in Western blot applications with human samples.DPYSL2, also known as collapsin response mediator protein 2 (CRMP2), is essential for axonal growth and guidance in the nervous system. Phosphorylation of DPYSL2 at threonine 514 is known to regulate its function in cell signaling pathways, making this antibody an important tool for investigating the role of DPYSL2 in neuronal development and neurodegenerative diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-CRMP2/DPYSL2-T514 Antibody
SKU:
CABP0821
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TVTP A
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
1808
RRID
AB_2771034
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (CABP0821) at 1:1000 dilution or CRMP2/DPYSL2 antibody (CAB14570). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
