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Anti-Phospho-eIF2alpha-S51 Antibody (CABP0745)

SKU:
CABP0745
Product Type:
Antibody
Applications:
WB
IP
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
  • Metabolism Antibodies 3 Anti-Phospho-eIF2alpha-S51 Antibody CABP0745
  • Metabolism Antibodies 3 Anti-Phospho-eIF2alpha-S51 Antibody CABP0745
€329 - €419
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Description

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Antibody Name:Anti-Phospho-eIF2alpha-S51 Antibody
Antibody SKU:CABP0745
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around S51 of human eIF2Alpha (NP_004085.1).
Application:WB IP
Recommended Dilution:WB 1:1000 - 1:2000 IP 1:50 - 1:100
Reactivity:Human
Positive Samples:HeLa
Immunogen:A synthetic phosphorylated peptide around S51 of human eIF2Alpha (NP_004085.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SELS R
Gene ID:1965
Uniprot:P05198
Cellular Location:Cytoplasmic granule
Calculated MW:36kDa
Observed MW:38kDa
Anti-Phospho-eIF2alpha-S51 Antibody (CABP0745)Western blot analysis of extracts of HeLa cells, using Phospho-eIF2a-S51 pAb (CABP0745) at 1:2000 dilution or EIF2S1 antibody (CAB0764).HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-Phospho-eIF2alpha-S51 Antibody (CABP0745)Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2a-S51 pAb (CABP0745). Western blot was performed from the immunoprecipitate using Phospho-eIF2a-S51 pAb (CABP0745) at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37'C for 30 minutes after serum-starvation overnight.
Synonyms:EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, EIF2S1
Background:The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
UniProt Protein Function:eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.
UniProt Protein Details:

Protein type:RNA-binding; Translation initiation; Translation

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; nucleus; cytosol; eukaryotic translation initiation factor 2 complex

Molecular Function:protein binding; translation initiation factor activity; ribosome binding

Biological Process: regulation of translation initiation in response to stress; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression

NCBI Summary:The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
UniProt Code:P05198
NCBI GenInfo Identifier:124200
NCBI Gene ID:1965
NCBI Accession:P05198.3
UniProt Related Accession:P05198
Molecular Weight:315
NCBI Full Name:Eukaryotic translation initiation factor 2 subunit 1
NCBI Synonym Full Names:eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
NCBI Official Symbol:EIF2S1  
NCBI Official Synonym Symbols:EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha  
NCBI Protein Information:eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha
UniProt Protein Name:Eukaryotic translation initiation factor 2 subunit 1
UniProt Synonym Protein Names:Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
Protein Family:Eukaryotic translation initiation factor
UniProt Gene Name:EIF2S1  
UniProt Entry Name:IF2A_HUMAN

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