MMP9 Antibody [L51/82 (Formerly sold as S51-82)] is a mouse monoclonal antibody directed against MMP9, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF, IP and FCM. Reported reactivity: Human, Mouse and Rat. Supplied as IgG2a, purified by Protein G.
Matrix metalloproteinase 9 (MMP9) is an extracellular endopeptidase involved in the degradation of the extracellular matrix (ECM). In the central nervous system, MMP9 regulates synaptic remodeling, neuroinflammation, and blood-brain barrier permeability. Elevated MMP9 activity has been observed in neurodegenerative diseases such as Alzheimer’s disease, multiple sclerosis, and amyotrophic lateral sclerosis (ALS). MMP9 contributes to neuronal injury by promoting ECM breakdown, facilitating leukocyte infiltration, and activating pro-inflammatory cytokines. MMP9 is synthesized as a latent proenzyme and requires proteolytic cleavage for activation. Its expression is tightly regulated at the transcriptional level and by endogenous inhibitors such as TIMPs (tissue inhibitors of metalloproteinases). Targeting MMP9 activity offers therapeutic potential for preserving blood-brain barrier integrity, reducing neuroinflammation, and limiting tissue damage in neurodegenerative conditions. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, IHC, ICC/IF, IP, FCM
Antibody Isotype:
IgG2a
Purification:
Protein G Purified
Concentration:
1 mg/ml
Reactivity:
Human, Mouse, Rat
Recommended Dilution:
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Specificity:
Detecs ~92kDa and ~82kDa (pro and active forms).
Guarantee:
12 months from date of dispatch
Immunogen:
Fusion protein amino acids 1-708 (full length) of rat MMP9
Immunogen Species:
Rat
Accession Number:
NP_112317.1
Gene ID:
2123-08-25 00:00:00
Swiss-Prot:
P50282
Cellular Localization:
Extracellular Matrix
Alternative Names:
MMP9, MMP-9, Matrix metalloproteinase 9, CLG4B, 82kDa matrix metalloproteinase-9, Collagenease type 4 beta, GELB, Macrophage gelatinase, MANDP2, Type V collagenase, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B
References:
1. Hirose Y., et al. (2008) Am J Hum Genet. 82(5): 1122-1129. 2. Coussens L.M., et al. (2002) Science 295: 2387-2391. 3. Sternlicht M.D., et al. (1999) Cell 98: 137-146. 4. Vu T.H., et al. (1998) Cell 93: 411-422. 5. Nagase H., et al. (1990) Biochemistry 29: 5783-5789.
