The Phospho-eIF4EBP1-T70 Monoclonal Antibody (CABP1334) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated through a rigorous process using hybridoma technology, specifically recognizes the phosphorylated form of eIF4EBP1 and is validated for use in various experimental techniques, including Western blotting and immunohistochemistry.eIF4EBP1 is a key regulator of protein translation and is often dysregulated in cancer and other diseases characterized by abnormal cell growth. Phosphorylation of eIF4EBP1 at T70 is known to enhance its inhibitory effect on translation initiation, making it a critical target for research into mechanisms of tumorigenesis and potential therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-eIF4EBP1-T70 Monoclonal Antibody
SKU:
CABP1334
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC53785
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TKTP PR
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BP-1, 4EBP1, 4E-BP1, PHAS-I, Phospho-eIF4EBP1-T70
Positive Sample:
293T treated with FBS, NIH/3T3
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
13kDa
Observed MW:
15-20kDa
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purification Method
Affinity purification
Gene ID
1978
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells using Phospho-eIF4EBP1-T70 Rabbit mAb (CABP1334) at 1:2000 dilution. 293T cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-eIF4EBP1-T70 Rabbit mAb (CABP1334) at 1:2000 dilution. NIH/3T3 cells were treated with CIP at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
