Western blot analysis of extracts of C6 cells, using Phospho-EZH2/KMT6-T311 antibody at 1:1000 dilution. C6 cells were treated by AICAR (0.5 mM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
The Phospho-EZH2/KMT6-T311 Polyclonal Antibody (CABP1269) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically targets the phosphorylated form of EZH2 at threonine 311, allowing for precise detection and analysis in human samples.EZH2, a member of the Polycomb group proteins, is known for its role in gene repression through histone methylation. The phosphorylation of EZH2 at T311 has been linked to its oncogenic function in various cancer types, making it a crucial biomarker for assessing disease progression and potential therapeutic targets.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
Phospho-EZH2/KMT6-T311 Polyclonal Antibody
SKU:
CABP1269
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
KNTE T
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
Purification Method
Affinity purification
Gene ID
2146
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C6 cells, using Phospho-EZH2/KMT6-T311 Rabbit pAb (CABP1269) at 1:1000 dilution. C6 cells were treated with AICAR (0. 5 mM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
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