The Phospho-Histone H2AX-S139 Antibody (CABP0099) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific for detecting phosphorylation of histone H2AFX at serine 139, a well-known marker of DNA double-strand breaks.Histone H2AFX phosphorylation at serine 139 plays a key role in the cellular response to DNA damage, initiating signaling cascades that promote repair processes and regulate cell cycle checkpoints. This antibody is validated for use in various applications, including Western blotting, immunoprecipitation, and immunofluorescence, allowing for detailed analysis of DNA damage signaling pathways in different experimental systems.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-Histone H2AX-S139 Antibody
SKU:
CABP0099
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TQAS Q
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:400 - 1:5000
IF/ICC
1:100 - 1:500
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
H2A.X, H2A/X, H2AFX, Phospho-Histone H2AX-S139
Positive Sample:
NIH/3T3 treated with UV, C6 treated with UV
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
15kDa
Observed MW:
17kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Purification Method
Affinity purification
Gene ID
3014
RRID
AB_2771168
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H2AX-S139 Rabbit pAb (CABP0099) at 1:400 dilution. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Western blot analysis of lysates from C6 cells using Phospho-Histone H2AX-S139 Rabbit pAb (CABP0099) at 1:400 dilution. C6 cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-Histone H2AX-S139 Rabbit pAb (CABP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using Phospho-Histone H2AX-S139 Rabbit pAb (CABP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of 293T(treated with UV) and 293T(untreated) cells using Phospho-Histone H2AX-S139 Rabbit pAb (CABP0099) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
