The Phospho-IRS1-S636/639 Antibody (CABP0551) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for phosphorylation at serine 636/639 on IRS1 and is validated for use in Western blot applications. It allows for the detection and analysis of phosphorylated IRS1 in human samples, making it an essential component of studies in the fields of diabetes, metabolism, and signal transduction.IRS1 is a key mediator of insulin signaling, playing a crucial role in glucose metabolism and insulin sensitivity. Phosphorylation of IRS1 at serine 636/639 has been linked to insulin resistance and impaired insulin signaling, making it a relevant target for research into conditions such as type 2 diabetes and metabolic disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-IRS1-S636/639 Antibody
SKU:
CABP0551
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MSPK SV
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
Purification Method
Affinity purification
Gene ID
3667
RRID
AB_2771218
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-IRS1-S636/639 Rabbit pAb (CABP0551) at 1:500 dilution. 293T cells were treated with IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
