Western blot analysis of extracts of C2C12 cells, using Phospho-IRS1-S1101 antibody (CABP0954) at 1:1000 dilution. C2C12 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-IRS1-S1101 Polyclonal Antibody (CABP0954) is a high-quality antibody developed for reliable detection and analysis of target proteins. Phosphorylation of IRS1 at this site plays a critical role in insulin signaling pathways, making this antibody essential for studies related to metabolic disorders such as diabetes.This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of IRS1 at serine 1101, researchers can investigate the downstream effects on insulin signaling and glucose metabolism in various cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-IRS1-S1101 Polyclonal Antibody
SKU:
CABP0954
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
HSSE T
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
Purification Method
Affinity purification
Gene ID
3667
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C2C12 cells, using Phospho-IRS1-S1101 Rabbit pAb (CABP0954) at 1:1000 dilution. C2C12 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
