The Phospho-JAK2 (Y1007) Polyclonal Antibody (CABP0373) is a vital tool for researchers studying the JAK2 signaling pathway in various biological processes. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it a reliable choice for Western blot applications. By targeting the phosphorylated form of JAK2 at tyrosine 1007, this antibody allows for precise detection and quantification of this specific post-translational modification in cells.JAK2, a member of the Janus kinase family, is a key player in signal transduction pathways involved in cell proliferation, differentiation, and immune response. Phosphorylation at tyrosine 1007 is known to modulate the activity of JAK2, influencing downstream signaling events.
Research into the role of this phosphorylation event is essential for understanding its implications in diseases such as cancer, hematologic disorders, and inflammatory conditions.With its ability to target and detect phosphorylated JAK2 at tyrosine 1007, the Phospho-JAK2 (Y1007) Polyclonal Antibody (CABP0373) is a valuable asset for investigators seeking to unravel the complexities of JAK2 signaling in health and disease. Its applications in immunology, oncology, and molecular biology make it a versatile tool for exploring the role of JAK2 phosphorylation in various physiological and pathological contexts.
Product Name:
Phospho-JAK2-Y1007 Rabbit Polyclonal Antibody
SKU:
CABP0373
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y1007 of human JAK2 (NP_004963.1).
This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
3717
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts from HT29 cells using Phospho-JAK2-Y1007 antibody (CABP0373).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
