Western blot analysis of HeLa, using Phospho-KAP1/TRIM28-S824 antibody (CABP1393) at 1:5000 dilution. HeLa cells were treated by Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-KAP1/TRIM28-S824 Monoclonal Antibody (CABP1393) is a high-quality antibody developed for reliable detection and analysis of target proteins. This post-translational modification of the protein is involved in various cellular processes, including DNA damage response and gene silencing.Raised in mice, this antibody shows high specificity for human samples and has been validated for use in Western blot and immunofluorescence applications. It specifically recognizes the phosphorylated form of KAP1/TRIM28 at serine 824, allowing for precise detection and analysis in different cell types.The phosphorylation of KAP1/TRIM28 at serine 824 has been linked to the regulation of chromatin structure and gene expression, making it a key player in gene regulation and cell signaling pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-KAP1/TRIM28-S824 Monoclonal Antibody
SKU:
CABP1393
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC59356
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
LSSQ E
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa treated with Etoposide, NIH/3T3 treated with Etoposide
Cellular Localization:
Nucleus.
Calculated MW:
79kDa/89kDa
Observed MW:
117kDa
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
Purification Method
Affinity purification
Gene ID
10155
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-KAP1/TRIM28-S824 Rabbit mAb (CABP1393) at 1:5000 dilution. HeLa cells were treated with Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-KAP1/TRIM28-S824 Rabbit mAb (CABP1393) at 1:5000 dilution. NIH/3T3 cells were treated with Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
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