Anti-Phospho-MAP2K1-S298 Antibody (CABP0063)
- SKU:
- CABP0063
- Product Type:
- Antibody
- Applications:
- WB
- IP
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-MAP2K1-S298 Antibody |
| Antibody SKU: | CABP0063 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S298 of human MAP2K1 (NP_002746.1). |
| Application: | WB IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, C2C12, C6 |
| Immunogen: | A synthetic phosphorylated peptide around S298 of human MAP2K1 (NP_002746.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PLSS Y |
| Gene ID: | 5604 |
| Uniprot: | Q02750 |
| Cellular Location: | Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body |
| Calculated MW: | 40kDa/43kDa |
| Observed MW: | 43kDa |
 | Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (CABP0063) at 1:1000 dilution or MAP2K1 antibody (CAB12687). HeLa cells were treated by PMA/TPA (200 nM) at 37'C for 15 minutes after serum-starvation overnight or treated by ATP(5 mM) at 30'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (CABP0063) at 1:1000 dilution. C2C12 cells were treated by CIP(20uL/400ul) at 37'C for 1 hour or treated by ATP(5 mM) at 30'C for 1 hour. C6 cells were treated by CIP(20uL/400ul) at 37'C for 1 hour or treated by ATP(5 mM) at 30'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Anti-Phospho-MAP2K1-S298 Antibody (CABP0063). Western blot was performed from the immunoprecipitate using Anti-Phospho-MAP2K1-S298 Antibody (CABP0063) at a dilition of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37'C for 30 minutes after serum-starvation overnight. |
| Synonyms: | CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MAP2K1 |
| Background: | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. |
| UniProt Protein Function: | MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. |
| UniProt Protein Details: | Protein type:EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Kinase, protein; Protein kinase, STE; STE group; STE7 family Chromosomal Location of Human Ortholog: 15q22.1-q22.33 Cellular Component: dendrite cytoplasm; Golgi apparatus; microtubule; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; perikaryon; cell cortex; cytosol; axon; perinuclear region of cytoplasm; late endosome; cytoplasm; plasma membrane; microtubule organizing center; nucleus Molecular Function:MAP kinase kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; Ras GTPase binding; receptor signaling protein tyrosine phosphatase activity; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; ATP binding; protein kinase activity Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; protein heterooligomerization; negative regulation of homotypic cell-cell adhesion; response to glucocorticoid stimulus; stress-activated MAPK cascade; cell motility involved in cell locomotion; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; chemotaxis; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; neuron differentiation; negative regulation of cell proliferation; positive regulation of RNA elongation from RNA polymerase II promoter; small GTPase mediated signal transduction; vesicle transport along microtubule; response to axon injury; cell cycle arrest; toll-like receptor 4 signaling pathway; Golgi inheritance; epidermal growth factor receptor signaling pathway; mitosis; fibroblast growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; melanosome transport; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; keratinocyte differentiation; cell proliferation; regulation of stress-activated MAPK cascade; regulation of vascular smooth muscle contraction; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; response to oxidative stress; positive regulation of Ras protein signal transduction; cell motility; positive regulation of cell differentiation; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration Disease: Noonan Syndrome 1; Cardiofaciocutaneous Syndrome 3 |
| NCBI Summary: | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q02750 |
| NCBI GenInfo Identifier: | 400274 |
| NCBI Gene ID: | 5604 |
| NCBI Accession: | Q02750.2 |
| UniProt Related Accession: | Q02750 |
| Molecular Weight: | 393 |
| NCBI Full Name: | Dual specificity mitogen-activated protein kinase kinase 1 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase kinase 1 |
| NCBI Official Symbol: | MAP2K1Â Â |
| NCBI Official Synonym Symbols: | CFC3; MEK1; MKK1; MAPKK1; PRKMK1Â Â |
| NCBI Protein Information: | dual specificity mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; ERK activator kinase 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) |
| UniProt Protein Name: | Dual specificity mitogen-activated protein kinase kinase 1 |
| UniProt Synonym Protein Names: | ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1 |
| UniProt Gene Name: | MAP2K1Â Â |
| UniProt Entry Name: | MP2K1_HUMAN |
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