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Western blot analysis of HeLa, using Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb (CABP1503) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

Phospho-Myosin Light Chain 2-T18/S19 Monoclonal Antibody (CABP1503)

Datasheet MSDS
SKU CABP1503
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human
Host Species Rabbit
Synonyms LC20, MLC2, MRLC1, MYRL2, MLC-2C, MMIHS4, Phospho-Myosin Light Chain 2-T18/S19
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Description

system_update_altDatasheetsystem_update_altMSDS

Phospho-Myosin Light Chain 2-T18/S19 Monoclonal Antibody (CABP1503)

The Phospho-Myosin Light Chain 2-T18/S19 Monoclonal Antibody (CABP1503) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through a monoclonal production process, shows high specificity and sensitivity towards the phosphorylated form of myosin light chain 2 at threonine 18 and serine 19 residues.Myosin light chain 2 is a key regulator of actin-myosin interactions in muscle contraction and cell motility. Phosphorylation of myosin light chain 2 at T18/S19 is known to play a role in various cellular processes, including smooth muscle contraction, cell migration, and cytokinesis.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:Phospho-Myosin Light Chain 2-T18/S19 Monoclonal Antibody
SKU:CABP1503
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC62066
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:RATS NV
Tested Applications:WB ELISA
Recommended Dilution:
WB1:2000 - 1:7000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:LC20, MLC2, MRLC1, MYRL2, MLC-2C, MMIHS4, Phospho-Myosin Light Chain 2-T18/S19
Positive Sample:HeLa treated by Calyculin A
Cellular Localization:Cell Cortex, Cytoplasm, Cytosol, Myofibril, Z Disc.
Calculated MW:20kDa
Observed MW:18kDa

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID10398
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb (CABP1503) at 1:6000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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