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Western blot analysis of HeLa, using Phospho-Myosin Light Chain 2-T18 Rabbit mAb (CABP1439) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

Phospho-Myosin Light Chain 2-T18 Monoclonal Antibody (CABP1439)

Datasheet MSDS
SKU CABP1439
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human
Host Species Rabbit
Synonyms LC20, MLC2, MRLC1, MYRL2, MLC-2C, MMIHS4, Phospho-Myosin Light Chain 2-T18
$173 - $603

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Description

system_update_altDatasheetsystem_update_altMSDS

Phospho-Myosin Light Chain 2-T18 Monoclonal Antibody (CABP1439)

The Phospho-Myosin Light Chain 2-T18 Monoclonal Antibody (CABP1439) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using advanced technology, specifically targets the phosphorylated form of myosin light chain 2 at threonine 18. It is highly reactive with human, mouse, and rat samples, and has been validated for use in various applications including Western blotting and immunofluorescence.Myosin light chain 2 is a key component of the cytoskeleton and is involved in the regulation of cellular contraction and movement. Phosphorylation of threonine 18 plays a crucial role in modulating the activity of myosin light chain 2 and is essential for processes such as cell migration, adhesion, and cytokinesis.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:Phospho-Myosin Light Chain 2-T18 Monoclonal Antibody
SKU:CABP1439
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC62064
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:ATSN V
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:LC20, MLC2, MRLC1, MYRL2, MLC-2C, MMIHS4, Phospho-Myosin Light Chain 2-T18
Positive Sample:HeLa treated with Calyculin A
Cellular Localization:Cell Cortex, Cytoplasm, Cytosol, Myofibril, Z Disc.
Calculated MW:20kDa
Observed MW:18kDa

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID10398
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-Myosin Light Chain 2-T18 Rabbit mAb (CABP1439) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
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