Anti-Phospho-NFKBIA-S32 Antibody (CABP0731)
- SKU:
- CABP0731
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| Antibody Name: | Anti-Phospho-NFKBIA-S32 Antibody |
| Antibody SKU: | CABP0731 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S32 of human I?BAlpha (NP_065390.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa |
| Immunogen: | A synthetic phosphorylated peptide around S32 of human I?BAlpha (NP_065390.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HDSG L |
| Gene ID: | 4792 |
| Uniprot: | P25963 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 35kDa |
| Observed MW: | 39kDa/41kDa |
 | Western blot analysis of extracts of HeLa cells, using Phospho-IkBalpha-S32 antibody (CABP0731) at 1:1000 dilution. HeLa cells were treated by TNF-a (20 ng/mL) at 37'C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of extracts of HeLa cells, using Phospho-IkBalpha-S32 pAb (CABP0731) at 1:1000 dilution or IKB alpha antibody (CAB11397).HeLa cells were treated by TNF-a (20 ng/mL) at 37'C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Immunohistochemistry of paraffin-embedded human colon using Phospho-IkBalpha-S32 Rabbit pAb (CABP0731) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using Phospho-IkBalpha-S32 Rabbit pAb (CABP0731) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using Phospho-IkBalpha-S32 Rabbit pAb (CABP0731) at dilution of 1:100 (40x lens). |
| Synonyms: | IKBA, MAD-3, NFKBI, IKB alpha, NFKBIA, EDAID2 |
| Background: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. |
| UniProt Protein Function: | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| NCBI Summary: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P25963 |
| NCBI GenInfo Identifier: | 126682 |
| NCBI Gene ID: | 4792 |
| NCBI Accession: | P25963.1 |
| UniProt Secondary Accession: | P25963,B2R8L6, |
| UniProt Related Accession: | P25963 |
| Molecular Weight: | 35,609 Da |
| NCBI Full Name: | NF-kappa-B inhibitor alpha |
| NCBI Synonym Full Names: | NFKB inhibitor alpha |
| NCBI Official Symbol: | NFKBIAÂ Â |
| NCBI Official Synonym Symbols: | IKBA; MAD-3; NFKBIÂ Â |
| NCBI Protein Information: | NF-kappa-B inhibitor alpha |
| UniProt Protein Name: | NF-kappa-B inhibitor alpha |
| UniProt Synonym Protein Names: | I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3 |
| Protein Family: | NF-kappa-B inhibitor |
| UniProt Gene Name: | NFKBIAÂ Â |
| UniProt Entry Name: | IKBA_HUMAN |
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