The Phospho-eNOS-S1177 Antibody (CABP0421) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for the phosphorylated form of NOS3 at Serine 1177, making it ideal for detecting and analyzing phosphorylation events in Western blot experiments.NOS3, also known as endothelial nitric oxide synthase, is essential for regulating blood vessel relaxation and blood flow, and dysfunction in NOS3 activity has been implicated in various cardiovascular diseases such as hypertension and atherosclerosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-eNOS-S1177 Antibody
SKU:
CABP0421
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TQSF S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
eNOS, ECNOS, Phospho-eNOS-S1177
Positive Sample:
Jurkat treated with Calyculin A, HeLa treated with IGF-1
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
4846
RRID
AB_2771372
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-eNOS-S1177 Rabbit pAb (CABP0421). Jurkat cells were treated with Calyculin A. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
Western blot analysis of lysates from HeLa cells, using Phospho-eNOS-S1177 Rabbit pAb (CAB1548). HeLa cells were treated with IGF-1 (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
