Western blot analysis of various lysates, using Phospho-PDK1/PDPK1-S241 antibody (CABP1357) at1:2000 dilution. PC-3 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-PDK1/PDPK1-S241 Monoclonal Antibody (CABP1357) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, exhibits high specificity and sensitivity in detecting phosphorylated PDK1 in human samples and is suitable for use in various applications, including Western blotting.PDK1, a pivotal kinase in the PI3K signaling cascade, plays a crucial role in cell growth, survival, and metabolism. Phosphorylation at serine 241 is known to enhance PDK1 activity, leading to the phosphorylation and activation of downstream targets such as Akt.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-PDK1/PDPK1-S241 Monoclonal Antibody
SKU:
CABP1357
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC57184
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ANSF V
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Enables 3-phosphoinositide-dependent protein kinase activity; phospholipase activator activity; and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway; regulation of protein kinase activity; and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection; cytosol; and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma.
Purification Method
Affinity purification
Gene ID
5170
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Phospho-PDK1/PDPK1-S241 Rabbit mAb (CABP1357) at 1:2000 dilution. PC-3 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 120s.
