The Phospho-PLC gamma 2 (PLCG2)-Y759 Antibody (CABP0785) is a high-quality antibody developed for reliable detection and analysis of target proteins. PLCG2 is a key enzyme involved in cellular signaling pathways, particularly in immune cells. This antibody, generated in rabbits, is highly specific to the phosphorylated form of PLCG2 and has been validated for use in various experimental techniques such as Western blotting.Phosphorylation of PLCG2 at tyrosine 759 has been linked to its activation and downstream signaling cascades, making it a crucial event in immune responses and inflammatory processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-PLC gamma 2 (PLCG2)-Y759 Antibody
SKU:
CABP0785
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RMYV D
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cytoplasm, Cytosol, Extracellular Exosome, Perinuclear Region Of Cytoplasm, Plasma Membrane, Ruffle Membrane.
Calculated MW:
148kDa
Observed MW:
150kDa
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
Purification Method
Affinity purification
Gene ID
5336
RRID
AB_2771439
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Raji cells, using Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb (CABP0785) at 1:2000 dilution. Raji cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
