The Phospho-PLN-S16 Antibody (CABP0907) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for the phosphorylation of PLN at serine 16 and is validated for use in various applications, including Western blot and immunohistochemistry.Phospholamban (PLN) is a critical regulator of cardiac muscle contraction, and its phosphorylation at serine 16 is known to relieve its inhibitory activity on the sarcoplasmic reticulum Calcium-ATPase (SERCA) pump, leading to enhanced calcium reuptake and improved cardiac function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-PLN-S16 Antibody
SKU:
CABP0907
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RAST I
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PLB, CMD1P, CMH18, Phospho-PLN-S16
Positive Sample:
C2C12 cells and C6 cells were treated with CIP, 293F cells transfected with PLN were treated with λ-PP
Cellular Localization:
Endoplasmic Reticulum, Mitochondrial Membrane, Mitochondrion, Perinuclear Region Of Cytoplasm.
Calculated MW:
6kDa
Observed MW:
12KkDa/17kDa/35kDa
The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.
Purification Method
Affinity purification
Gene ID
5350
RRID
AB_2863820
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-PLN-S16 Rabbit pAb (CABP0907) at 1:1000 dilution. C2C12 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour. C6 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from 293F cells using Phospho-PLN-S16 Rabbit pAb (CABP0907) at 1:1000 dilution incubated overnight at 4℃. 293F cells transfected with PLN were treated with λ-PP mixed solution (1μL) at 30℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
