The Phospho-PPP2CA/PPP2CB-Y307 Antibody (CABP0927) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity in detecting phosphorylated Y307 residues of PPP2CA and PPP2CB in human samples.Validation studies have shown that this antibody is suitable for Western blot applications, enabling precise analysis of the phosphorylation status of PPP2CA and PPP2CB in various cell types. The ability to target specific phosphorylation sites on these proteins makes this antibody particularly useful for investigations in signal transduction pathways, protein regulation, and cell signaling.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-PPP2CA/PPP2CB-Y307 Antibody
SKU:
CABP0927
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PDYF L
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PPP2CA/PPP2CB, Phospho-PPP2CA/PPP2CB-Y307
Positive Sample:
HeLa treated with Calyculin A, NIH/3T3 treated with Calyculin A
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
Purification Method
Affinity purification
Gene ID
5515 5516
RRID
AB_2863839
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb (CABP0927) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100nM) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated with Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
