Anti-Phospho-PRKAA1-T183/PRKAA2-T172 pAb Antibody (CABP0883)
- SKU:
- CABP0883
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-Phospho-PRKAA1-T183/PRKAA2-T172 Antibody |
| Antibody SKU: | CABP0883 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T183 of human PRKAA1 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | 293T, C6 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T183 of human PRKAA1 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 5562/5563 |
| Uniprot: | Q13131/P54646 |
| Cellular Location: | |
| Calculated MW: | 64kDa/65kDa/62kDa |
| Observed MW: | 62kDa |
 | Western blot analysis of extracts of various cell lines, using Phospho-AMPKa1-T183/AMPKa2-T172 antibody (CABP0883) at 1:1000 dilution.293T and C6 cells were treated by ATP(5 mM) at 30'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
| Synonyms: | AMPKa1/AMPKa2 |
| Background: | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
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