Anti-PTPN6 Antibody (CAB1446)

Antibody Name:	Anti-PTPN6 Antibody
Antibody SKU:	CAB1446
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human PTPN6 (NP_002822.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, MCF7, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human PTPN6 (NP_002822.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LNCS DPTS ERWY HGHM SGGQ AETL LQAK GEPW TFLV RESL SQPG DFVL SVLS DQPK AGPG SPLR VTHI KVMC EGGR YTVG GLET FDSL TDLV EHFK KTGI EEAS GAFV YLRQ PYYA TRVN AADI ENRV LELN KKQE SEDT AKAG FWEE FESL QKQE VKNL HQRL EGQR PENK GKNR YKNI LPFD HSRV ILQG RDSN IPGS DYIN ANYI KNQL LGPD ENAK TYIA SQGC LEAT VNDF WQMA WQEN SRVI VMTT REVE KGRN KCVP YWPE VGMQ RAYG PYSV TNCG EHDT TEYK LRTL QVSP L
Gene ID:	5777
Uniprot:	P29350
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	63kDa/67kDa/70kDa
Observed MW:	68kDa

Synonyms:

HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1, CTCN6, PTPN6

Background:

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.

UniProt Protein Function:	Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
NCBI Summary:	The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	P29350
NCBI GenInfo Identifier:	131469
NCBI Gene ID:	5777
NCBI Accession:	P29350.1
UniProt Secondary Accession:	P29350,Q969V8, Q9UK67, A8K306, G3V0F8,
UniProt Related Accession:	P29350
Molecular Weight:	64 kDa
NCBI Full Name:	Tyrosine-protein phosphatase non-receptor type 6
NCBI Synonym Full Names:	protein tyrosine phosphatase, non-receptor type 6
NCBI Official Symbol:	PTPN6
NCBI Official Synonym Symbols:	HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
NCBI Protein Information:	tyrosine-protein phosphatase non-receptor type 6
UniProt Protein Name:	Tyrosine-protein phosphatase non-receptor type 6
UniProt Synonym Protein Names:	Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; PTP-1C; Protein-tyrosine phosphatase SHP-1; SH-PTP1
Protein Family:	Tyrosine-protein phosphatase non-receptor
UniProt Gene Name:	PTPN6

	Western blot analysis of extracts of various cell lines, using PTPN6 antibody (CAB1446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using PTPN6 antibody (CAB1446). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Phospho-PTPN6-Y536 Antibody (CABP0440)	SHP-1 (Phospho-Tyr536) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-PTPN6-Y564 pAb Antibody (CABP0787)	SHP-1 Colorimetric Cell-Based ELISA Kit
Anti-SHP1 Antibody (CAB19111)
PTPN6 Antibody (PACO01496)
Phospho-PTPN6 (Y536) Antibody (PACO03047)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)