The Phospho-Raf1-S259 Antibody (CABP0497) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it suitable for a range of applications, including Western blotting. By specifically binding to the phosphorylated form of Raf1 at serine 259, this antibody allows for precise detection and analysis of Raf1 activation in various cell types.Raf1 is a crucial kinase involved in cell growth, differentiation, and survival, making it a potential target for cancer research and drug development. Phosphorylation at serine 259 plays a role in regulating Raf1 activity and downstream signaling cascades, highlighting the importance of studying this post-translational modification.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-Raf1-S259 Antibody
SKU:
CABP0497
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
STST P
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Purification Method
Affinity purification
Gene ID
5894
RRID
AB_2771491
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-Raf1-S259 Rabbit pAb (CABP0497) at 1:1000 dilution. HeLa cells were treated with 10% FBS after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-Raf1-S259 pAb (CABP0497). Western blot was performed from the immunoprecipitate using Phospho-Raf1-S259 pAb (CABP0497) at a dilution of 1:1000. HeLa cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
