The Phospho-Raf1-S621 Monoclonal Antibody (CABP1011) is a high-quality antibody developed for reliable detection and analysis of target proteins. RAF1 is a key player in the MAPK signaling pathway, which is involved in cell proliferation, differentiation, and survival. Phosphorylation of RAF1 at serine 621 is known to activate its kinase activity, leading to downstream signaling events.This antibody, generated in rabbits, shows high specificity and sensitivity in detecting phosphorylated RAF1 at serine 621 in human samples. It is validated for use in Western blot applications, allowing for precise analysis of RAF1 phosphorylation levels in various cell types and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Phospho-Raf1-S621 Monoclonal Antibody
SKU:
CABP1011
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC1556
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SASE P
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Purification Method
Affinity purification
Gene ID
5894
RRID
AB_2863900
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C6 cells, using Phospho-Raf1-S621 Rabbit mAb (CABP1011) at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Western blot analysis of lysates from HeLa cells, using Phospho-Raf1-S621 Rabbit mAb (CABP1011) at 1:1000 dilution. HeLa cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
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