Western blot analysis of various lysates, using Phospho-Smad2-S245/S250/S255 antibody (CABP1338) at1:2000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-Smad2-S245/S250/S255 Monoclonal Antibody (CABP1338) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using an innovative monoclonal antibody technology, is highly specific and sensitive for detecting phosphorylation at serine residues 245, 250, and 255 of SMAD2.This antibody is ideal for use in Western blotting, immunoprecipitation, and immunofluorescence applications, allowing for the detection and analysis of phosphorylated SMAD2 in various cell types and tissue samples. Its high reactivity with human samples makes it a reliable tool for studies in cell signaling, developmental biology, and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-Smad2-S245/S250/S255 Monoclonal Antibody
SKU:
CABP1338
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC53851
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TGSP AELS PTTL SPV
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cytoplasm, Cytosol, Homomeric Smad Protein Complex, Nucleoplasm, Nucleus.
Calculated MW:
52kDa
Observed MW:
60kDa
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Purification Method
Affinity purification
Gene ID
4087
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from293T cells using Phospho-Smad2-S245/S250/S255 Rabbit mAb (CABP1338) at1:2000 dilution. 293T cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60 s.
![Anti-Phospho-Smad2 (Ser255) [R02-8F2] Monoclonal Antibody (AGMB05059)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/276344/677472/anti-phospho-smad2-ser255-r02-8f2-monoclonal-antibody-agmb05059__76624.1773032678.jpg?c=2 "Anti-Phospho-Smad2 (Ser255) [R02-8F2] Monoclonal Antibody (AGMB05059)")