The Phospho-Smad3-S423/S425 Monoclonal Antibody (CABP0727) is a high-quality antibody developed for reliable detection and analysis of target proteins. Smad3 is a critical protein involved in the TGF-β signaling pathway, playing a significant role in cell growth, differentiation, and apoptosis.This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By specifically targeting the phosphorylated form of Smad3, researchers can effectively study the activation and regulation of the TGF-β signaling pathway in various cell types.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-Smad3-S423/S425 Monoclonal Antibody
SKU:
CABP0727
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0170
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SSVS
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000 - 1:3000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa treated with TGF-β, C2C12 treated with TGF-β3, C6 treated with TGF-β3
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
48kDa
Observed MW:
52kDa
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.
Purification Method
Affinity purification
Gene ID
4088
RRID
AB_2863813
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-Smad3-S423/S425 Rabbit mAb (CABP0727) at 1:1000 dilution incubated overnight at 4℃. C2C12 cells and C6 cells were treated with TGF-β3 (50 ng/mL) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20 s.
Western blot analysis of lysates from HeLa cells using Phospho-Smad3-S423/S425 Rabbit mAb (CABP0727) at 1:1000 dilution incubated overnight at 4℃. HeLa cells were treated with TGF-β (50 ng/mL) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
Immunohistochemistry analysis of paraffin-embedded Rat ovary tissue using Phospho-Smad3-S423/S425 Rabbit mAb (CABP0727) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Phospho-Smad3-S423/S425 Rabbit mAb (CABP0727) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-Smad3-S423/S425 Rabbit mAb (CABP0727) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
