The Phospho-STAT3-Y705 Monoclonal Antibody (CABP0705) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot and immunohistochemistry applications.Stat3 activation at tyrosine 705 is known to play a key role in regulating gene expression related to cell proliferation, survival, and differentiation. Dysregulation of Stat3 signaling has been implicated in various diseases including cancer, inflammation, and autoimmune disorders.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-STAT3-Y705 Monoclonal Antibody
SKU:
CABP0705
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC50831
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
APYL K
Tested Applications:
WBIHC-PELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IF-P
1:50 - 1:200
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
APRF, HIES, ADMIO, ADMIO1, Phospho-STAT3-Y705
Positive Sample:
NIH/3T3 treated with TNF-α
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
88kDa
Observed MW:
79kDa/88kDa
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.
Purification Method
Affinity purification
Gene ID
6774
RRID
AB_2863810
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT3-Y705 Rabbit mAb (CABP0705) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-STAT3-Y705 Rabbit mAb (CABP0705) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Phospho-STAT3-Y705 Rabbit mAb (CABP0705) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat thymus tissue using Phospho-STAT3-Y705 Rabbit mAb (CABP0705) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
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