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Western blot analysis of C6, using Phospho-Stathmin 1-S25 Rabbit mAb (CABP1440) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

Phospho-Stathmin 1-S25 Monoclonal Antibody (CABP1440)

Datasheet MSDS
SKU CABP1440
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Rat
Host Species Rabbit
Synonyms Lag, SMN, OP18, PP17, PP19, PR22, LAP18, C1orf215, Phospho-Stathmin 1-S25
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Description

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Phospho-Stathmin 1-S25 Monoclonal Antibody (CABP1440)

The Phospho-Stathmin 1-S25 Monoclonal Antibody (CABP1440) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced against the phosphorylated form of Stathmin-1, is highly specific and sensitive for detecting phosphorylated Stathmin-1 in human samples.Phosphorylation of Stathmin-1 at S25 is known to influence cell cycle progression, cell migration, and cytoskeleton organization, making it a key player in cancer progression and metastasis.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.

Product Name:Phospho-Stathmin 1-S25 Monoclonal Antibody
SKU:CABP1440
Size:20μL, 100μL
Reactivity:Human, Rat
Clone Number:ARC62298
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:RASG Q
Tested Applications:WB ELISA
Recommended Dilution:
WB1:1000 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:Lag, SMN, OP18, PP17, PP19, PR22, LAP18, C1orf215, Phospho-Stathmin 1-S25
Positive Sample:C6 treated with PMA
Cellular Localization:Cytoplasm, Cytoskeleton.
Calculated MW:17kDa
Observed MW:17kDa

This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID3925
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from C6 cells, using Phospho-Stathmin 1-S25 Rabbit mAb (CABP1440) at 1:1000 dilution. C6 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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