The Phospho-STK11-T189 Antibody (CABP0603) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to the phosphorylated form of STK11 at threonine 189 and is validated for use in various applications, including Western blotting.STK11, also known as LKB1, is a tumor suppressor protein that is frequently mutated in various cancers, most notably in lung and cervical cancers. Phosphorylation of STK11 at threonine 189 has been linked to the regulation of its activity, affecting downstream signaling cascades involved in cell cycle regulation and apoptosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-STK11-T189 Antibody
SKU:
CABP0603
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
GGTL K
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PJS, LKB1, hLKB1, Phospho-STK11-T189
Positive Sample:
A-431 treated with FBS
Cellular Localization:
Cytoplasm, Membrane, Mitochondrion, Nucleus.
Calculated MW:
49kDa
Observed MW:
54kDa
The protein encoded by this gene is a serine/threonine kinase that regulates cell polarity and energy metabolism and functions as a tumor suppressor. Mutations in this gene have been associated with the autosomal dominant Peutz-Jeghers syndrome, as well as with skin, pancreatic, and testicular cancers.
Purification Method
Affinity purification
Gene ID
6794
RRID
AB_2771579
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-STK11-T189 Rabbit pAb (CAB2122). A-431 cells were treated with 10% FBS for after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020).
