The Phospho-TAK1-S439 Monoclonal Antibody (CAB9437) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. RRID Gene ID 6885 Swiss Prot Synonym CSCF; FMD2; TAK1; MEKK7; TGF1a; Phospho-TAK1-S439
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-TAK1-S439 Monoclonal Antibody
SKU:
CAB9437
Size:
100μL, 20μL
Reactivity:
Mouse
Clone Number:
ARC2714
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CSCF, FMD2, TAK1, MEKK7, TGF1a, Phospho-TAK1-S439
Positive Sample:
C2C12 treated with TGF-β
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
67kDa
Observed MW:
70-82kDa
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. RRID Gene ID 6885 Swiss Prot Synonym CSCF; FMD2; TAK1; MEKK7; TGF1a; Phospho-TAK1-S439
Purification Method:
Affinity purification
Gene ID:
6885
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using (CAB9437) at 1:1000 dilution. C2C12 cells were treated with TGF-β (10 ng/ml) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
