The Phospho-Tau (Thr212/Ser214) Polyclonal Antibody (CABP1126) is specifically designed for research involving phosphorylated tau protein, a key player in the pathogenesis of Alzheimer's disease and other neurodegenerative disorders. The antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.Phosphorylation of tau at Thr212 and Ser214 sites has been implicated in the formation of neurofibrillary tangles, a hallmark of Alzheimer's disease pathology. By targeting these phosphorylation sites, researchers can investigate the role of tau protein in disease progression and identify potential therapeutic targets for the treatment of neurodegenerative disorders.
The Phospho-Tau (Thr212/Ser214) Polyclonal Antibody enables precise detection and analysis of phosphorylated tau protein in neuronal cell lines, brain tissues, and other relevant samples. Its application in neuroscience research makes it a valuable tool for studying the molecular mechanisms underlying neurodegeneration and exploring novel therapeutic approaches for Alzheimer's disease and related conditions.
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Purification Method:
Affinity purification
Gene ID:
4137
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brain, using Phospho-Tau-Thr212/Ser214 Rabbit pAb (CABP1126) at 1:1000 dilution.Mouse brain were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
