Anti-GSK3Beta Antibody (CAB6164)

Antibody Name:	Anti-GSK3Beta Antibody [KO Validated]
Antibody SKU:	CAB6164
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GSK3Beta (NP_001139628.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:1000 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, HeLa, 22Rv1, U-251MG

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GSK3Beta (NP_001139628.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PWTK VFRP RTPP EAIA LCSR LLEY TPTA RLTP LEAC AHSF FDEL RDPN VKLP NGRD TPAL FNFT TQEL SSNP PLAT ILIP PHAR IQAA ASTP TNAT AASD ANTG DRGQ TNNA ASAS ASNS T
Gene ID:	2932
Uniprot:	P49841
Cellular Location:	Cell membrane, Cytoplasm, Nucleus
Calculated MW:	46kDa/48kDa
Observed MW:	46kDa

Synonyms:

GSK3B, gsk-3Beta

Background:

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation
UniProt Protein Details:	Protein type:Protein kinase, CMGC; EC 2.7.11.26; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; GSK family; GSK subfamily Chromosomal Location of Human Ortholog: 3q13.3 Cellular Component: axon; beta-catenin destruction complex; cell soma; centrosome; cytoplasm; cytosol; dendritic shaft; dendritic spine; growth cone; lipid raft; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane; ribonucleoprotein complex Molecular Function:ATP binding; beta-catenin binding; integrin binding; ionotropic glutamate receptor binding; kinase activity; NF-kappaB binding; p53 binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau protein binding; tau-protein kinase activity; ubiquitin protein ligase binding Biological Process: aging; axonogenesis; cell migration; circadian rhythm; dopamine receptor signaling pathway; epithelial to mesenchymal transition; ER overload response; establishment of cell polarity; fat cell differentiation; genetic imprinting; glycogen metabolic process; hippocampus development; myoblast fusion; negative regulation of apoptosis; negative regulation of dendrite morphogenesis; negative regulation of glycogen biosynthetic process; negative regulation of MAP kinase activity; negative regulation of neuron maturation; negative regulation of NFAT protein import into nucleus; negative regulation of nitric-oxide synthase activity; negative regulation of protein binding; negative regulation of protein complex assembly; organ morphogenesis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of axon extension; positive regulation of cell-matrix adhesion; positive regulation of GTPase activity; positive regulation of neuron apoptosis; positive regulation of peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein binding; positive regulation of protein catabolic process; positive regulation of protein complex assembly; positive regulation of protein export from nucleus; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein export from nucleus; re-entry into mitotic cell cycle; regulation of microtubule-based process; regulation of neuronal synaptic plasticity; response to activity; response to drug; response to estradiol stimulus; response to insulin stimulus; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin
NCBI Summary:	The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
UniProt Code:	P49841
NCBI GenInfo Identifier:	20455502
NCBI Gene ID:	2932
NCBI Accession:	P49841.2
UniProt Secondary Accession:	P49841,Q9BWH3, Q9UL47, D3DN89,
UniProt Related Accession:	P49841
Molecular Weight:	48,034 Da
NCBI Full Name:	Glycogen synthase kinase-3 beta
NCBI Synonym Full Names:	glycogen synthase kinase 3 beta
NCBI Official Symbol:	GSK3B
NCBI Protein Information:	glycogen synthase kinase-3 beta
UniProt Protein Name:	Glycogen synthase kinase-3 beta
UniProt Synonym Protein Names:	Serine/threonine-protein kinase GSK3B (EC:2.7.11.1)
Protein Family:	Glycogen synthase kinase
UniProt Gene Name:	GSK3B
UniProt Entry Name:	GSK3B_HUMAN

	Western blot analysis of extracts of various cell lines, using GSK3b antibody (CAB6164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts from normal (control) and GSK3b knockout (KO) HeLa cells, using GSK3b antibody (CAB6164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using GSK3b antibody (CAB6164) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of 22Rv1 cells, using 3 ug GSK3b antibody (CAB6164). Western blot was performed from the immunoprecipitate using GSK3b antibody (CAB6164) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-GSK3Beta Antibody (CAB11360)	Human GSK 3 beta / Glycogen synthase kinase-3 beta ELISA Kit
Anti-GSK3Beta Antibody (CAB11578)	Human GSK3 beta (Glycogen Synthase Kinase 3 Beta) CLIA Kit (HUES01084)
Anti-GSK3Beta Antibody (CAB16868)	Human Glycogen synthase kinase-3 beta (GSK3B) ELISA Kit
Anti-GSK3Beta Antibody (CAB2081)	GSK3beta (Phospho-Ser9) Colorimetric Cell-Based ELISA Kit
Anti-GSK3Beta Antibody (CAB3174)[KO Validated]	GSK3beta Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)