The Phospho-TP53 (S392) Polyclonal Antibody (CABP0860) is a valuable tool for researchers studying the TP53 protein, a tumor suppressor involved in regulating cell growth and preventing cancer development. This antibody, raised in rabbits, is highly specific to phosphorylated TP53 at serine 392 and is validated for use in Western blot applications. It enables detection and analysis of TP53 phosphorylation in various cell types, providing important insights into the role of this protein in cancer biology.TP53 is a key player in maintaining genomic stability and preventing the proliferation of damaged cells. Phosphorylation at serine 392 is known to impact TP53 stability and function, influencing its ability to induce cell cycle arrest or apoptosis in response to cellular stress.
Understanding the phosphorylation status of TP53 is essential for unraveling the complex regulatory mechanisms that govern cell growth and survival, particularly in the context of cancer development.By utilizing the Phospho-TP53 (S392) Polyclonal Antibody in their research, scientists can delve deeper into the molecular pathways controlled by TP53 and its phosphorylation events. This antibody offers a reliable tool for studying the dynamic changes in TP53 activity and identifying potential therapeutic targets for manipulating TP53 function in cancer treatment strategies.
Product Name:
Phospho-p53-S392 Rabbit Polyclonal Antibody
SKU:
CABP0860
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S392 of human p53 (NP_000537.3).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p53-S392 Rabbit pAb (CABP0860) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 1s.
