The PID1 Antibody (CAB13220) is a high-quality antibody developed for reliable detection and analysis of target proteins. Involved in several processes, including mitochondrion morphogenesis; negative regulation of phosphate metabolic process; and positive regulation of macromolecule metabolic process. Located in cytoplasm.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PID1 Antibody
SKU:
CAB13220
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 25μg-100μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PCLI1, NYGGF4, P-CLI1, HMFN2073, PID1
Positive Sample:
U-87MG, Mouse brain, Mouse kidney, Mouse liver, Rat brain
Cellular Localization:
Cytoplasm.
Calculated MW:
28kDa
Observed MW:
27kDa
Involved in several processes, including mitochondrion morphogenesis; negative regulation of phosphate metabolic process; and positive regulation of macromolecule metabolic process. Located in cytoplasm.
Purification Method
Affinity purification
Gene ID
55022
RRID
AB_2760073
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PID1 Rabbit pAb (CAB13220) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Immunoprecipitation analysis of 25 μg extracts of Mouse liver cells using 3 μg PID1 antibody (CAB13220). Western blot was performed from the immunoprecipitate using PID1 antibody (CAB13220) at a dilution of 1:1000.
